thermo fisher Gibco™ B-27™ Supplement (50X), serum free
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The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic, post-natal, and adult hippocampal and other CNS neurons. B-27 Supplement is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer. 描述
The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic, post-natal, and adult hippocampal and other CNS neurons. B-27 Supplement is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.
B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.
For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.
View publications using Gibco neurobiology products at our Gibco CellCite.
For Research Use Only. Not for use in diagnostic procedures.
图表
Primary rat cortex neurons cultured in B-27 Supplement
Immunoflorescence detection of primary neuronal cells stained with mouse anti-MAP2 antibody (green). Nuclei are stained with DAPI (blue).
Primary rat Hippocampus neurons cultured in B-27 Supplement
Immunoflorescence detection of primary neuronal cells stained with mouse anti-MAP2 antibody and singular presence of astrocyte as detected by rabbit anti-GFAP marker.
Rat cortical neurons cultured in Neurobasal Medium with B-27 Supplement
Primary rat cortex neurons (E-18) cultured in Neurobasal Medium supplemented with B-27 Supplement for 21 days. At Day 21 cells were fixed and stained with the MAP2 antibody and DAPI.规格
浓缩: 50 X 产品类型: Supplement 无菌: Sterile 分类: Serum-Free 运输条件: Dry Ice 经过测试: Endotoxin, Performance, Sterility, Mycoplasma 产品线: B-27™ 形式: Liquid 有效期: 12 Months 细胞类型: Neural Cells 来源: Serum-Free pH: 6 to 8 内容及储存
Store in freezer (-5 to -20°C) and protect from light.
手册和实验方案
產品簡介
